Problemas de salud mental

Resumen

Plant fructokinase (FRK) guarantees the growth and development of higher plants by participating in carbohydrate metabolism. In this study, a new fructokinase, OsFRK3, was identified using bioinformatics analysis, enzyme assay, bacterial growth assay, and yeast complementation test. Then, we created <i>OsFRK3</i> knockout transgenic lines (<i>osfrk3</i>-1 and <i>osfrk3</i>-2) by the CRISPR/Cas9 technology. We found that the 1000-grain weight decreased notably (approximately -3.6% and -6.1%, respectively) in <i>osfrk3</i>-1 and <i>osfrk3</i>-2. Evidently decreased grain width, grain thickness, and endosperm filling rate were detected in the <i>osfrk3</i> mutants (<i>osfrk3</i>-1 and <i>osfrk3</i>-2) compared with those of the WT. In addition, the content of seed total starch was significantly decreased by 3.42 and 4.80% in <i>osfrk3</i> lines, compared with that in the WT. The level of maltose was significantly reduced in the mutants, while that of sucrose and fructose was obviously increased in the mutants. The transcript levels of <i>OsGBSS</i>1, <i>OsBEIIb</i>, <i>OsPFP1β</i>, and <i>OsAGPL1</i> were significantly decreased in the <i>osfrk3</i> mutants. These results suggest that OsFRK3 may positively regulate the accumulation of starch through influencing the sugar metabolism.