First Detection of the CTXM-15 Producing Escherichia coli O25-ST131 Pandemic Clone in Ecuador

Resumen

Our aim was identify of the pandemic B2-ST131 <i>Escherichia coli</i> clone by to the Institute Pasteur and Achtman scheme, and investigate the resistance profile phenotypic-genotypic, with identification of class 1 integron. Of thirty-five ESBL-producing isolates recovered of patients with diagnosis of urinary tract infections (UTI), six <i>E. coli</i> strains serotype O25 were identified with resistance antimicrobial to several groups of antibiotics such as broad-spectrum cephalosporins, fluoroquinolones and aminoglycosides, harboring <i>blaSHV, blaCTX-M</i> genes in all isolates and <i>blaTEM</i> in two isolates. Sequencing of <i>blaCTX-M</i> revealed CTX-M-15 in all strains. The PMQR <i>aac(6&acute;)-Ib-cr</i> and <i>qnrB19</i> genes were presented in five and four isolates respectively, AMEs genes <i>aac(6&acute;)-Ib</i> and <i>aac(3)-IIa</i> were presented in strain amikacin-gentamicin-resistant. Sequencing of the variable regions of the class 1 integron revealed <i>dfrA</i> and <i>aadA</i> genes cassette. The analysis of multilocus sequence typing (MLST) confirms the presence of the pandemic B2-ST131 <i>E. coli</i> clone by Achtman scheme in all ST43 isolates obtained by of the Institute Pasteur scheme. The results presented herein, reveal the presence of B2-ST131 <i>E. coli</i> clone in Ecuador, disseminated in hospitals and community settings.