La presencia de PDGFRβ incrementa la proliferación, migración y capacidad tumorigénica en cultivos primarios de cáncer de mama

Resumen

Introduction: Breast cancer is the most frequent malignant tumor in women. Previous evidence has shown the presence of PDGFRb in breast cancer with some prognostic issues, but the carcinogenic role has been described partially. Methods: Tumoral behavior was evaluated in cells with and without PDGFRb expression. This evaluation was realized in two phases; in vitro by using proliferation and migration tests and in vivo, in a murine model where tumorigenic capacity was evaluated by the growth rate and tumoral weight. Results: Proliferative rate and migration capacity were significantly higher in cellular lines with PDGFRb expression in relation to silenced lines. The silence in the PDGFRb receptor diminished the MAPK’s activity. Finally tumorigenic capacity in murine models dictated by time to tumor appearance, growth rate, were higher in cell lines with PDGFRb+. Conclusion: PDGFRb participates actively in proliferation, migration and tumorigenesis of primary cultures of breast cancer.